
"Every fish that swims leaves a little of itself behind — skin cells, mucus, waste. That shed DNA floating in the water is environmental DNA, and we can read it."

Environmental DNA (eDNA) is genetic material organisms shed into their environment — via skin cells, mucus, feces, gametes, and decomposition. In water, this DNA persists for hours to days before degrading (UV, microbes, pH). By filtering a water sample and sequencing the captured DNA, we can detect which species were present without ever capturing or seeing them. Two broad approaches: (1) single-species (targeted) assays using quantitative PCR (qPCR) to detect one species of interest; (2) multi-species (metabarcoding) using high-throughput sequencing to survey whole communities from a single sample. eDNA has become a core tool in modern biodiversity monitoring and is increasingly accepted in regulatory contexts.
